Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Expressing, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Binding Assay

Journal: bioRxiv

Article Title: Fentanyl blockade of K + channels contribute to Wooden Chest Syndrome

doi: 10.1101/2025.01.17.633656

Figure Lengend Snippet: a. RT-PCR assays with total RNAs from micro-dissected spinal C3-5 ventral horn tissue and whole brain with primer pairs specific to each of the 8 genes encoding all EAG subtypes of K + channel subunits. Selective enrichment for the expression of only Eag (Kv10.1, Kv10.2)- and Erg (Kv11.1, Kv11.2, Kv11.3)-subtypes, with little to no expression detected for Elk (Kv12.1, Kv12.2, Kv12.3)-subtype transcripts. By contrast, all EAG transcripts were readily detected from whole brain total RNA. Parallel control assays with first-strand reactions performed without reverse transcriptase (-) were negative. All assays repeated 4X with similar results. b. Detection of individual Eag/Erg transcripts in single phrenic motoneurons by in situ hybridization with RNAscope probes. Phrenic motoneuron in spinal cervical segments C3-5 were identified by retrograde labeling by injections of fluorogold (FG) in the lateral diaphragm, and immunolabeling for choline acetyltransferase (ChAT). Shown are examples of phrenic motoneurons (FG + , ChAT + ), co-labeled with RNAscope probes specific to each of the Eag/Erg -subtype transcripts. Quantitation revealed nearly all phrenic motoneurons expressed Eag transcripts [Kv10.1 (∼96%) and Kv10.2 (85%)], and ∼60% expressed two of the three Erg transcripts [Kv11.1 (66%), Kv11.3 (60%), Kv11.2 (22%)] (see Methods for details of quantitation).

Article Snippet: Following probe hybridizations, slides underwent sequential incubations with RNAscope Multiplex Detection Reagents (ACD #323110) consisting of FL v2 Amp 1, for 30 mins at 40°C, with a 5 min PBS wash following each step.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Reverse Transcription, In Situ Hybridization, RNAscope, Labeling, Immunolabeling, Quantitation Assay